急性白血病血管内皮细胞活性功能的研究

SOME ACTIVE FACTORS RELEASED BY ENDOTHELIAL CELLS IN ACUTE LEUKEMIA

应用放射免疫法、Ｌａｕｒｅｌｌ＇ｓ５火箭免疫电泳法、交叉免疫电泳法和发色底物法对急性白血病（急白）患者中由血管内皮细胞产生释放的一些重要活性因子如ｖｏｎＷｉｌｌｅｂｒａｎｄ因子相关抗原（ｖＷＦ：Ａｇ）、前列环素、血浆纤维结合蛋白（Ｆｎ）、血浆纤溶酶原激活物（ｔ－ＰＡ）和抑制物的水平和活性变化进行研究，并探讨这些活性因子与急白病情变化及疗效转归的关系，揭示血管内皮细胞在急白中的地位。结果发现急白患者血浆中ｖＷＦ：Ａｇ、血浆６－酮－前列腺素Ｆ＿（１α）和ｔ－ＰＡ显著高于正常水平，在有出血倾向患者中有特征性变化，病情好转后逐渐趋向正常。急白在发生感染、出血或慢性粒细胞白血病急变时其血浆Ｆｎ明显低于正常水平。急白患者ｖＷＦ和Ｆｎ质量也有变化。

In recent years,it is known that endothelial cells play an important role in the genesis of some dis-eases,but there are only few papers dealing with the role of endothelial cells in leukemia. In this study some of the active factors released by the endothelial cells in patients with acute leukemia such as von Willebrand factor,prostaglandin,fibronectin, tissue-type plasminogen activator and its inhibitor were determined. It was found that the levels of these factors changed significantly in patients with acute leukemia, as compared with those in nonnal controls, especially in cases with bleeding tendency and in-fection. The possible mechanism and the clinical significance were discussed.