应用丁型肝炎病毒27肽抗原检测丁型肝炎病毒抗体

APPLICATION OF SYNTHETIC 27 OLIGOPEPTIDE OF HDV ANTIGEN FOR DETECTING SERUM ANTO-HD IN HBV INFECTED SUBJECTS IN CHONGQING

采用中科院上海生化所合成丁型肝炎病毒（ＨＯＶ）抗原２７肽酶联法，检测重庆地区乙型肝炎病毒（ＨＢＶ）感染者中血清丁型肝炎病毒抗体（抗－ＨＤ）结果：３００例献血员中１例阳性（此人ＡＬＴ较正常升高２倍）１１３例甲型肝炎、５８例非乙型肝炎均为阴性；ＨＢＶ标记阳性者８８２例中抗－ＨＤ（＋）１０６例（１２．０２％），其中乙型肝炎表面抗原携带者１３／４１０例（３．１７％）、急性肝炎１１／７６例（１４．４％）、慢性迁延性肝炎１／１３例（７．６９％）、慢性活动性肝炎２２／１２１例（１７．６８％）、重型肝炎１７／８６例（１９．７７％）、肝硬化２３／７８例（２９．４９％）、原发性肝细胞癌１９／９８例（１９．３９％）。结果与已往报告基本一致。与ＡｂｂｏｔｔＥＩＡ试剂相符率为７４／７８例（９４．９％）。采用天然丁型肝炎病毒抗原（ＨＯＡｇ）与之竞争抗－ＨＤ，４份阳性转为阴性，２份阴性结果不变，提示本ＨＤＶ抗原合成肽具有天然ＨＤＡｇ活性，可特异识别抗－ＨＤ，是一新的、稳定、安全、可靠的诊断用试剂。

synthetic HDAg 27 peptide which was selected and designed by the authors and synthesized by Shanghai Institute of Biochemistry, Chinese Academy of Science was used with ELISA method to de-tect serum anti HD in HBV infected subjects in Chongqing.Anti HD was positive in one of 300 blood donors and was negative in all of 113 cases of hepatitis A and 58 cases of hepatitis non-B. Anti HD was positive in 106 out of 882 cases with positive HBV marker(1202%),among which anti HD was positive 1n 3.17%(13/410)of HBsAg carrier, 14.4%(11 /76) of acute hepatitis,7.6%(1/ 13) of chronic per-sistent hepatitis, 17.68%(22 / 121)of chronic active hepatitis,19.77%(17 / 86) of severe hepatitis,29.49%(23 / 78)of liver cancer and 19.39% (19/ 98)of primary hepatic cancer. These results coincided with those of previous reports.The coincidence rate was 94.9%(74/ 78)when compared with Abbott EIA kit.When the natural HDAg was used to compete anti HD in four anti HD positive and two anti HD negative serum specimens,anti HD was negative in all specimens. It is shown that the HDAg 27peptide has natural HDAg activity capable of being recognized by natural anti HD and is a new diagnostic agent being more simple,save,stable and reliable.