丙型肝炎病毒抗体检测的诊断价值

THE DIAGNOSTIC VALUE OF ANTI-HCV TEST IN HCV INFECTION

用国产第二代丙型肝炎病毒抗体（抗－ＨＣＶ）检测试剂盒检测了２９６例临床血清标本以及２８例供血者血清标本。并用聚合酶链反应（ＰＣＲ）方法检测了这些标本的丙型肝炎病毒（ＨＣＶ）ＲＮＡ，以了解抗－ＨＣＶ检测对ＨＣＶ感染的诊断价值。同时探讨其在供血员筛选中的应用价值。结果在临床标本中，抗－ＨＣＶ阳性者有８１．１％ＨＣＶＲＮＡ阳性。抗－ＨＣＶ阴性者有２１．７％ＨＣＶＲＮＡ阳性。但是２８例抗－ＨＣＶ阴性的供血者ＨＣＶＲＮＡ全部阴性。提示：抗－ＨＣＶ阳性对于ＨＣＶ感染的诊断价值很高。临床怀疑为ＨＣＶ感染而进行检测的血清标本即使抗－ＨＣＶ阴性，也不能除外ＨＣＶ感染。用抗－ＨＣＶ筛选供血者可能使供血中ＨＣＶＲＮＡ阳性率大大降低。

wo hundred and ninety-six samples of patient serum and 28 samples of donor serum were tested for anti-HCV with second generation domestic made testing kit,and for HCV RNA with PCR assay. The aim of this study is to assess the diagnostic value of anti-HCV test in HCV infection and to discuss its value in screening of donors.The results were as follows HCV RNA was detected in 81.6% of the anti-HCV positive patient samples and in 21.7 % of the anti-HCV negative patient samples.HCV RNA was not detected in 28 anti-HCV negative donors.These results reveals that positive anti-HCV is of high diagnostic value in HCV infection. Anti-HCV negative could not exclude HCV infection for samples suspected of HCV infection. However,screening donors with anti-HCV probably decreases HCV RNA positive rate considerably.