抗类风湿关节炎36kD和33kD抗体对类风湿关节炎诊断意义的研究

EVALUATION OF ANTI-RHEUMATOID ARTHRITIS 36 kD AND ANTI-33kD ANTIBODIES IN THE DIAGNOSIS OF RHEUMATOID ARTHRITIS

我们自艾氏小鼠腹水癌细胞（Ｅｈｒｌｉｃｈａｓｃｉｔｅｃｅｌｌｓ）提取抗原，进行免疫印迹法检测，发现８８例类风湿关节炎（ＲＡ）患者中有２８例抗３６ｋＤ（分子量为３６０００）抗体阳性，占３１．８％．而在１００例其他结缔组织病患者及４０例正常对照组中均无１例出现阳性。然而，用上述方法提取的抗原，以免疫印迹法检测时，抗ＲＡ３３ｋＤ抗体或抗ＲＮＰ抗体均可与３３ｋＤ区带反应，说明抗３３ｋＤ抗体对诊断ＲＡ的特异性不高。结果表明抗３６ｋＤ抗体是ＲＡ的特异性抗体。如果同时出现抗３６ｋＤ及抗３３ｋＤ抗体，则更加有利于诊断。

y using soluble nuclear extracts from Ehrlich mice ascite cells,immunoblot analysis showed reactivity of rheumatoid arthritis(RA) serum with a 36 kD band.It was found that 28 of 88 patients with RA had anti-RA 36 kD antibody (31.8%),but there was no reactivity in the serum samples from100 patients with other connective tissue diseases and 40 normal controls.However,the nuclear antigen mentioned above could react with either anti-RA 33 kD antibody or anti-RNP antibodv in the 33 kD band.Thus,anti-RA 33 kD antibody was not a specific antibody for RA. Anti-RA 36 kD antibody ppeared to be a marker antibody in RA. If anti-RA 36 kD and anti-33 kD antibodies appeared in a serum sample simultaneously,it might be more valuable for the diagnosis of RA.