转化生长因子β_1对人原代白血病细胞的诱导分化作用

THE EFFECTS OF RECOMBINANT HUMAN TRANSFORMING GROWTH FACTOR BETA ON INDUCING DIFFERENTIATION OF FRESH LEUKAEMIA CELLS IN ACUTE MYELOGENOUS LEUKEMIA M_5

为观察转化生长因子β＿１（ＴＧＦ－β＿１）对人原代白血病细胞是否存在诱导分化作用，本实验以５例初发末治的急性单核细胞白血病患者骨髓细胞为体外模型，用ＴＧＦ－β＿１１０ｎｇ／ｍｌ作用６天后，可见细胞形态与诱导前相比有明显变化，原始单核细胞、幼稚单核细胞比例明显下降，成熟细胞比例升高；仅１例可见虽有原始单核细胞比例的下降，但幼稚单核细胞百分比上升，成熟单核细胞比例变化不大；所有病例诱导前后均为过氧化物酶弱阳性，α－ＮＳＥ阳性并被氟化钠抑制，表明诱导后细胞仍属单核细胞系，并且不同病例的细胞对ＴＧＦ－β＿１的反应性各异。

By using fresh leukemia cells from 5 cases of acute myelogenous leukemia M_5as in vitro model,we investigated the effects of recombinant human transforming growth factor beta_1(rhTGF-β_1)on differentiation induction of fresh leukemia cells. The results indicated that after 6 days ofinduction with TGF-β_1in a concentrationof 10 ng / ml,leukemia cells in 5 AML-M_5 patients differentiated obvious-ly to maturation. The proportion of monoblasts and premonocytes was reduced,while that of mature mononuclear cells elevated.Following administration of TGF-β_1,α-nonspecific esterase(α-NSE),whose expression could be inhibited by sodium fluoride,remained positive and peroxidase(POX) was shown to be weakly positive These results demonstrated that TGF-β_1 may induce in vitro differentiation offresh leukaemia cells,but the reactions to TGF-β_1 may vary in different cases.