摘要
Chemically cross-linked hydrogels constitute a novel injectable tissue engineering material. At present, one of the key problems is to find an appropriate initiator. This study evaluated the cytotoxicity in vitro of a water-soluble redox initiating system consisting of ammonium persulfate (APS) and N, N, N′, N′-tetramethylethylenediamine (TEM- ED). Gelation time of PEG diacrylate macromer in phos- phate buffer saline solution was first adjusted to guarantee that the examined initiator concentrations are sufficiently high to trigger polymerization of macromers. NIH/3T3 fi- broblasts were employed to examine cytotoxicity via MTT measurements and optical microscopic observations. It has been found that the combined APS/TEMED system exhibits negative cooperative effect, for the underlying cytotoxicity is even lower than that of APS or TEMED at certain concen- trations.
Chemically cross-linked hydrogels constitute a novel injectable tissue engineering material. At present, one of the key problems is to find an appropriate initiator. This study evaluated the cytotoxicity in vitro of a water-soluble redox initiating system consisting of ammonium persulfate (APS) and N, N, N', N'-tetramethylethylenediamine (TEMED). Gelation time of PEG diacrylate macromer in phosphate buffer saline solution was first adjusted to guarantee that the examined initiator concentrations are sufficiently high to trigger polymerization of macromers. NIH/3T3 fibroblasts were employed to examine cytotoxicity via MTT measurements and optical microscopic observations. It has been found that the combined APS/TEMED system exhibits negative cooperative effect, for the underlying cytotoxicity is even lower than that of APS or TEMED at certain concentrations.
基金
the Nationa1 Natura1 Science Foundation of China(Grant Nos.2O174006,20221402,30271293 , 20374015)
the Key Grant of the Chinese Ministry of Education(Grant No.305004)
the Award Foundation for Young Teachers from the Ministry of Education
the 973 Project(Grant No.G1999054306-03)
the 863 Project(Grant No.2004AA215170)
the Science and Technology Developing Foundation of Shanghai (Grant Nos.04JC14019,02DJ14017).
关键词
组织工程
血管注射剂
水凝胶
初始系统
细胞毒素
tissue engineering
injectable biomaterial
hydrogel
initiator
cytotoxicity
