外源DNA通过花粉管通道法导入玉米的研究

Introduction of Exogenous DNA into Maize via Pollen Tube Pathway

利用花粉管通道技术,以抗虫的胰蛋白酶抑制剂基因(CpTI)为供体,以8个品种玉米为受体,进行外源DNA直接导入,获得了大量的转基因植株,通过对抗性植株的初步分子鉴定,证实外源CpTI基因存在并整合入玉米基因组中。

Exogenous DNA has transformed CpTI into maize by pollen tube pathway, and off springs have been obtained. These plants were carried on further screen of kanamycin and molecular test. Some of them were confirmed to be transgenic plants by assays of PCR. The molecular test proved that the foreign gene was inherited and expressed in maize plant.