氮芥和地塞米松对人角朊细胞血小板活化因子的抑制作用

Inhibitory Effect of Nitrogen mustard and dexamethasoue on Platelet Activated Factor Synthesis to Cultured human Keratinocytes

卡西霉素刺激原代培养人角朊细胞合成并释放血小板活化因子（ＰＡＦ），用洗涤兔血小板聚集法测定。结果表明，氮芥、地塞米松在 １０－９ｍｏｌ／Ｌ～ １０－５ｍｏｌ／Ｌ浓度范围内均能明显抑制角朊细胞ＰＡＦ生成，并与药物呈剂量和时间依赖关系，氮芥ＩＣ５０为６．３４ １０－９ｍｏｌ／Ｌ、地塞米松ＩＣ５０为１．００５ｘ １０－８ｍｏｌ／Ｌ。提示，氮芥和糖皮质激素抑制角遥远朊细胞ＰＡＦ生成很可能是其治疗银屑病机理的一部分。

We used the method of aggregation of washed rabbit platelet to study the effect of nitrogen mustard and dexamethasone on the synthesis of PAF from calcimycin (A23187) sit-' mulated human primary cultured keratinocytes and found that both nitrogen mustard and dexamcthasone could does -- and timedependently inhibit PAF synthesis. The ICSO of nitrogen mustard and dexamethasone are 6.34 X 10--u mol/L and 1.005 X 10--8 mol/L respectively. The results indicate that the inhibition of PAF synthesis might be part of tile mechanism of action of nitrogen mustard and dexamethasone therapy in psoriasis.