金银花中绿原酸的提取及检测

Extraction and Examination of Chlorogenic Acid from Flos Lonicerae

采用多种方法分别从金银花中提取绿原酸,用紫外光谱法和高效液相色谱法对其进行定性定量分析。同时用金银花乙醇提取物对大肠杆菌进行抗菌试验。绿原酸提取试验结果表明:用70%乙醇热回流提取是金银花中绿原酸提取的最佳方法,获得绿原酸的提取率高于其他方法,提取终产物与标准品的最高吸收峰均在324nm处,终产物中绿原酸的含量为43.35%。抗菌试验结果表明:金银花提取物具有较强的抑菌活性。

Optimization of the extracting method of chlorogenic acid from Flos Lonicerae was studied by several different methods. Two different ways of Ultraviolet (UV) and Visible Spectrophotometr and High Performance Liquid Chromatography (HPLC) have been carried out for the quantitative and qualitative determination of chlorogenic acid in Flos Lonicerae. Inhibition test against pathogenic bacteria has been done with Flos Lonicerae ethanol and chlorogenic acid. The results of chlorogenic acid extraction showed that chlorogenic acid tiptop absorb-apex was 324nm, with ethanol of 70% concentration extraction was remarkably superior to other methods. The content of chlorogenic acid with 70% ethanol extraction was 43.35%, so the ethanol with 70% concentration extraction was the optimum condition. The antibacterial action of chlorogenic acid on Escherichia in vitro Coliand extracted with 70% ethanol from Flos Lonicerae showed that Flos Lonicerae had strong antimicrobial activity.