四环素类抗生素残留的ELISA检测研究

Study on ELISA for Tetracyclines Residues in Animal Tissues

本试验通过间接竞争ELISA反应检测血液、肌肉及内脏组织中土霉素、四环素、金霉素的残留量,在50～300μg/kg浓度范围内,标准添加样品回收率81.3%～122.4%,变异系数2.7%～11.1%。本方法的建立。

With the development of animal husbandry, Tetracycline antibiotics (oxytetracycline, tetracycline and chlortetracyclin) have been widely used in feed industry as feed additives. It's likely to be left as a residues in tissues, milk and other food products with resulting risk to consumer. An enzyme-linked immunosorbent assay (ELISA) procedure for the analysis of TCs residues was established in this paper. The experiments shown that the limit of quantification was 100ug/ml, good recoveries were observed from 50 to 300μg/kg, with values of 81.3%～122.4%and CV% were 2.7%～11.1%.The advantages of the method were found to be rapid, simple and repeatable.