摘要
用改良酶联免疫斑点15试验(简称 M-Dot-ELISA)对麻风家内接触者(HC)血清进行了麻风杆菌特异性酚酚糖脂I(PGL-I)抗原检测。75例HC均经麻风杆菌明胶微粒凝集试验(MLPA)和酶联免疫吸附试验(ELISA)证实为麻风杆菌特异性抗体阳性者。结果表明:①75例HC中抗原阳性者16例,阳性率为21%。接触多菌型麻风(MB)者的抗原阳性率(28%)明显高于接触少菌型麻风(PB)者(0%),两者之间有极显著性差异( P <0.01 )。②抗体弱、强阳性组的抗原阳性率和抗原量之间亦有极显著差异(P<0.01)。③40例正常人及10例经MLPA和ELISA检测证实特异性抗体阳性的非麻风患者血清标本,抗原检测均阴性。
We report the results of detecting M. lepraespecific antigen--phenolic glycolipid I (PGL--I) with modified M-Dot-ELISA in sera from 75 cases of household contacts (HC)of leprosy patients, which were all seropositive by Gelatin Particle Agglutination Test (MLPA)and ELISA. The results indicate that: (1 ) 16/75 (21. 3 % ) are antigen positive. The rates of positivity in HC of MB patients are much higher than those in HC of PB patients,the difference (P < 0. 01 ) between them is significant. (2 ) Positive rates and levels of PGL-I in HCs are associated with levels of antibody against PGL--I in sera from HCs. There are significant differences in antigen positive rates and levels between weak and strong antibody positive groups of HC (P <0. 01). (3)The sera from 40 normal controls and 10 nonleprosy patients with positive anti--PGL--I antibody by MLPA and ELISA are antigen negative.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
1994年第2期67-69,共3页
Chinese Journal of Dermatology