大疱性类天疱疮230000和160000抗原的区域性分布

Regional Distribution of 230 000 and 160 000 Bullous Pemphigoid Antigens

利用免疫印迹技术筛选出２份大疱性类天疱疮（ＢＰ）血清，一份只和２３０ ０００分子结合，一份只和１６０ ０００分子结合，分别和人体２２处正常皮肤作间接免疫荧光（ⅡＦ），发现２３０ ０００抗原和１６０ ０００抗原的表达存在明显的区域性差异。２３０ ０００抗原在胸腹和大腿屈侧含量最高，头皮和足跖最低。１６０ ０００抗原在胸腹含量最高，头皮和颈部含量最低。２３０ ０００抗原和１６０ ０００抗原在同一部位的表达基本一致，但在腋窝、大腿伸侧和大腿屈侧三个部位明显高于后者。上述差异与临床上ＢＰ的好发部位有一定关联，靶抗原表达的区域性差异是造成自身免疫性皮肤病皮疹特异分布形式的一个重要原因。

Two sera,which recognize 230 000 BP antigen and 160 000 BP antigen respectively, were selected by Western blot. Twenty two skin pieces which were tak- en from different areas of cadavers were tested with the above sera by IIF. The results showed that there were regional variations on the expression of 230 000 antigen and 160 000 antigen. The concentration of 230 000 antigen was highest in the skin of upper chest, abdomen and the flexor aspect of thigh,and lowest in the scalp and planta. The concentration of 160 000 antigen was highest in the skin of upper chest and the abdomen,and lowest in the scalp and neck. There was hardly any difference on the same area between the two antigenic expression, but 230 000 antigen was markedly expressed than 160 000 antigen in several ar- eas. The regional variation of BP antigens reVeals some relation with the predilection of skin lesions in differ- ent areas. The regional variation of the target antigens may be a significant cause for disease specific distribu- tion of skin lesions of autoimmune dermatoses.