白念珠菌芽管特异性抗原的研究

Study of Germ Tube-Specific Antigens of Candida Albicans

运用ⅡＦ、ＳＤＳ－ＰＡＧＥ和免疫印迹技术，证实８株临床分离的白念珠菌芽管表面存在芽管特异性抗原。白念珠菌菌丝相胞壁提取物中含有比孢子相更多的、不同的蛋白成份；发现白念珠菌菌丝相胞壁提取物中有一分子量大约为３９ ０００蛋白抗原成份很可能是白念珠菌芽管特异性抗原；认为白念珠菌孢子相和其它念珠菌孢子相胞壁中有以隐蔽状态存在的芽管抗原成份。

ByⅡF,SDS-PAGE and Western blot technique,it was confirmed that there were germ tube-specific anti- gens on the surfaces of germ tubes of C. albicans which were isolated from clinical specimens. The mycelial- phase extract of C. albicans contained a more varied array of proteins than those of the yeast-phase ex- tract. We found a protein component (molecular weight, 39 000)in cell wall extracts of mycelial-phase, which may be a germ tube--specific antigen of C. albicans. It is our opinion that there may be germ tube antigens in a cryptic state not only in the cell walls of yeast-phase of C. albicans but in other candida species.