麻风患者治疗前后血清麻风杆菌特异性抗原和抗体的变化

Detection of PGL - I Antigen and Antibody Before and After Treatment in Leprosy Patients

用免疫斑点试验改良法（Ｍ－Ｄｏｔ－ＥＬＩＳＡ）和酶联免疫吸附试验（ＥＬＩＳＡ）对１０例多菌型麻风患者治疗前后的９９份血清进行了ＰＧＬ－Ｉ抗原和抗体检测。结果表明治疗前后及治疗中各段时间的抗原下降速度远快于抗体，二者之间有非常显著性差异（Ｕ＝９．０５，＞２．５８，Ｐ＜０，０１）。抗原量的下降以治疗后第１个月最快（７９．１４％），治疗第３个月时已下降９９％以上，这提示抗原的检测可用于监测多菌型麻风的早期疗效，在发现耐药和抗麻风新药筛选方面亦有应用价值。

We report the results of detecting anti-PGL-I antigen and antibody in sera from lepromatous leprosy patients before and after MDT with M-DotELISA and ELISA. The results indicated that the speed of antigen reducing in the sera of patients was much faster that that of antibody reducing after chemotherpy, the difference is very significant(U =9. 05, > 2. 58, P < 0. 01). The decreasing of PGL -1 antigen was fastest (79. 14 % )at the first month and was 99 % decreasing at the third months. It suggests that the detection of PGL-I antigen might be used for monitoring the early therapeutic effect in mutibacillary leprosy, finding out of drug - resistant M. leprae strains, and screening of new drugs for the treatment of leprosy.