紫外线辐射的尿刊酸对小鼠迟发型超敏反应抑制的研究

Suppression of Delayed Type Hypersensitivity Response to DNP_6OVA in Mouse by UV --Irradiated Urocanic Acid

建立了以小鼠耳厚的增加反映对ＤＮＰ６－ＯＶＡ抗原的迟发型超敏反应（ＤＴＨ）的动物模型，以观察经紫外线辐射的顺式尿刊酸（ｃｉｓ－ＵＣＡ）对小鼠ＤＴＨ的抑制程度。结果注射ｃｉｓ－ＵＣＡ的小鼠耳厚增加与对照组有显著性差异（尸＜０．０１），而注射反式尿刊酸（ｔｒａｎｓ－ＵＣＡ）的小鼠耳厚增加与对照无显著性差异（Ｐ＞０．０５）。表明在对ＤＮＰ６－ＯＶＡ的免疫应答中，ｃｉｓ－ＵＣＡ能够抑制细胞免疫反应。

In order to investigate the effects of cis-UCA on cell -mediated immunity, we established a murine model of DTH response to DNP6OVA which is indicated by the increasing in the ear thickness of the mouse after resensitization, compared with those of the mouse injected with 200 g cis - UCA and trans -UCA intraperitoneally (i. p. ). The average of the increasing in ear thickness of each group was 9. 1 1. 13 10-2 mm (n=8, the number of ears) for the group administrated with cis-UCA; 17. 45 0. 88 10 - 2 mm (n= 8) for the group administrated with trans-UCA ; 16. 43 + 1. 83 10 - 2 mm(n= 7) for control group. It is suggested that there are immunosuppression of DTH response to DNP6OVA in mouse administrated with ets-UCA and no effecs of trans-UCA on this response.