二甲基亚砜和羟乙基淀粉联合冷冻保护人骨髓造血细胞的实验研究

Experimental cryopreservation of human bone marrow cells in Dimethylsulfoxide and Hydroxyethyl Starch

在程控和直接存放于－８０℃冰箱两种降温方式下，采用联合冷冻保护液（５％二甲基亚砜＋６０ｇ／Ｌ羟乙基淀粉＋４０ｇ／Ｌ（白蛋白）冷冻保护人骨髓细胞，并与传统冷冻保护液（１０％二甲基亚砜＋１０％人血清）相比较。结果，粒细脑─巨噬细胞集落的回收率各组间的差异不显著，但在室温下，前者对造血细胞增殖能力的抑制较小。此外，直接存放于－８０℃冰箱可代替程控降温，前者具有简便、易规范化的特点。这些结果有利于我国骨髓移植工作的开展。

Fractionated human bone marrow cells could be successfully cryopreserved in a DMSO/HES/Albumin mixture prepared by a modified Ringer's solution as well as the conventional cryoprotectant (10% DMSO) with either-80℃ freezer or programmed freezer. However, the toxicity to suppress granulocyte-macrophage progenitors (CFU-GM) of the former was lower than that of the latter at room temperature, and-80℃ freezer with its advantages of simplicity and easy standardization could substitute for rate-contrulled freezer.