HLA-DRB1基因与螨性哮喘的相关性研究

Relationship between HLA-DRB1 gene and acarian asthma

目的探讨HLADRB1位点基因与螨性哮喘的相关性。方法分别对30例螨性哮喘患者、30例非螨性哮喘患者和30例正常对照者作吸入尘螨激发试验,采用序列特异性引物-聚合酶链反应(PCRSSP)方法分析螨性哮喘发作期HLADRB107、04和14等位基因在三组中的分布情况。结果螨性哮喘患者组DRB107等位基因频率(23.33%)较非螨性哮喘患者组(6.66%)和正常对照组(3.33%)均显著增高(χ2=7.94,P<0.01;χ2=12.27,P<0.01);DRB104和DRB114等位基因频率在螨性哮喘患者组(分别为6.66%、5.00%)较正常对照组(23.33%、23.33%)明显降低(χ2=7.94,P<0.01;χ2=9.93,P<0.01)。结论HLADRB107是螨性哮喘的遗传等位易感基因,DRB104和14基因可能在螨性哮喘的发生过程中具有保护作用。

Objective To investigate the relationship between HLA-DRB1 gene and acarian asthma. Methods Inhalation of dermatoph agoides pteronyssinus provocation test was performed among 30 patients with acarian asthma, 30 patients with non-acarian asthma and 30 normal controls. Polymer ase chain reactive-specific sequence primers(PCR-SSP) method was used in analy sing distribution of HLA-DRB1*07,*04 and*14 in the three groups. Results The allele frequency of DRB1*07 in the patients with acarian asthma(23.33%) was significantly higher than that in the patients with non-acarian asthma(6.66%,χ~2=7.94,P<0.01) and in the normal controls(3.33%,χ~2=12.27,P<0.01). The frequencies of DR B1*04 and DBB1*14 in the patients with acarian asthma were 6.66% and 5.00%, significantly lower than those in the normal controls(23.33%,χ~2=7.94,P<0 .01; 23.33%,χ~2=9.93,P<0.01), respectively. Conclusion HLA-DRB1*07 may be a susceptible alleles of a carian asthma. On the contrary, HLA-DRB1*04 and DRB1*14 genes may be the protective factors against acarian asthma.