替硝唑口贴片在健康者唾液中药物浓度测定及其药代动力学

Pharmacokinetics of tinidazole in saliva in healthy volunteers

目的:对口腔唾液中替硝唑浓度及其药代动力学参数进行研究。方法:以美国Beckman公司ODSC18(5μm, 4. 6mm×25cm)为色谱柱,流动相为甲醇—2. 33×10-3 mol/L冰醋酸水溶液(32∶68),流速1ml/min;柱温为室温;检测波长为310nm。结果:替硝唑贴片在唾液中的Tmax为( 1. 64±0. 94 ) h、Cmax为( 69. 23±11. 39)μg/ml、Ke为0. 059 6±0. 013 2、T1 /2为(11. 952±2. 374)h、AUC为(83. 42±11. 49)μg·h/ml、Clr为60. 64±8. 56、Vr为(2 704. 45±601. 42)ml。结论:该方法可用于临床替硝唑在唾液中药物浓度监测和药动学研究。

Objective:To study the pharmacokinetic paramet er s of tinidazole in saliva. Methods:Tinidazole was separated on U ltrasphere ODS (C 18 column, 5 μm,4.6 mm×25 cm)column with a mobile phas e of MeOH-2.33×10 -3 mol/L acetic acid 32∶68(v/v). The sample s were quantified with an ultraviolet detector operated at 310 nm. Resul ts:The saliva T max of the tinidazole tablets was (1.64±0.94) h,C max (69.23±11.39) μg/ml,K e 0.059 6±0.013 2,T 1/2 (11.952±2.374) h,Auc (83.42±11.49) μg·h/ml,Cl r 60.64± 8.56 and V r (2 704.45±601.42) ml respectively.Conclus ion:The method can be used for clinical monitoring of tinidazole in sali va.