中华眼镜蛇毒组分C与传统化疗药物体外抑制人胶质瘤株作用的对比研究

Comparative study of in vitro inhibitory effects on human glioma cell line between fraction C from Naja Naja atra venom and traditional chemotherapeutic drugs

目的研究中华眼镜蛇毒组分C(FC)体外对人胶质瘤细胞株U251的抑制作用。方法培养箱孵育U251与正常人胶质细胞;加入不同浓度的FC、阿霉素(ADM)和尼莫斯汀(ACNU)。采用细胞毒实验(MTT法),通过细胞生长抑制率评价FC、ACNU和ADM的细胞毒作用。结果FC对胶质瘤细胞的抑制作用突出,24h和48h的IC50分别为2.95μg/ml和2.21μg/ml,而ADM与ACNU远未达到此抑制率。FC对正常胶质细胞的24h、48hIC50分别为8.60μg/ml和6.61μg/ml,两细胞株间存在显著性差异(P<0.05)。结论人胶质瘤细胞对FC的细胞毒性作用是敏感的。FC对胶质瘤细胞的抑制作用优于ADM和ACNU。

Objective To investigate the cytotoxic effect of fraction C from Naja Naja atra venom (FC) on human glioma cell line (U251). Methods Normal human gliocyte and U251 were incubated with different concentrations of FC, adriamycin (ADM) and nimustine hydrochloride (ACNU). The cytotoxic effects of FC, ADM and ACNU on gliocyte and U251 were assessed according to the inhibition rate of cell growth by MTT rduction assay. Result The inhibitory effect of FC on U251 was obvious, with the inhibitory concentrations (IC50) of 2.95 and 2.21μg/ml at 24, 48 h respectively. Compared with the IC50 of 8.60 μg/ml and 6.61 μg/ml respectively for glial cell, and the difference was significant (P<0.05). Conclusion FC can inhibit the cell growth effectively and induce the apoptosis of U251 and is more effective in inhibiting the growth of glioma cells compared with ADM or ACNU.