PAAF诱导肠损伤的实验研究

Experimental Study on the Intestinal Harm Induced by PAAF in Rats

目的:探讨胰腺炎相关性腹水(PAAF)诱导的大鼠肠损伤作用及其相关机制.方法:以PAAF诱导的SD大鼠肠损伤模型为研究对象,将32只SD大鼠(雌雄不拘)随机分为两组,每组16只.A组(对照组):腹腔内注射生理盐水8 mL/只;B组(实验组):腹腔内注射PAAF 8 mL/只.于6、12 h分批处死大鼠,每时间点8只.测定血清内毒素(LPS)和血清肿瘤坏死因子-a(TNF-a)的浓度以及血白细胞(WBC)计数,大鼠呼吸频率(R)计数.测定小肠组织ATP酶的活性,测定肠系膜淋巴结的细菌移位率,于光镜下观察肠粘膜损伤情况并作等级比较.结果:同时间点比较:B组(实验组)血清LPS、TNF-a、WBC,呼吸频率(R),肠系膜淋巴结的细菌移位率,肠粘膜损伤程度均较A组(对照组)增高(P＜0.01～0.05),ATP酶活性显著降低(P＜0.01).结论:PAAF诱导的大鼠肠损伤明显.

Objective: To investigate the intestinal harm induced by pancreatitis associated ascetic fluid (PAAF) in rants and explore its mechanism. Methods: 32 Sprague-Dawley(SD) rats, male or female, were randomly divided into 2 groups: group A served as the control group and 8mL normal saline (NS) was injected into the peritoneal cavity; group B as the trial group, 8mL PAAF was injected. After peritoneal cavity injection the rats were put to death in 2 batches at 6h and 12h, 8 rats per batches. Levels of TNF-aLPSWBC in serum were measured. Mesenteric lymph node (MLN) was microbiologically studied.The activity of ATP enzyme in the intestinal tissues was measured.The pathological changes of intestines were observed by microscope.Results: The levels of TNF-aLPSWBC in serum,the incidence rates in bacterial translocation of MLN and the degree of intestinal harm were markedly elevated (P<0.01～0.05) and the activity of ATP enzyme of the intestinal tissues was decreased (P<0.05) in the group B compared to those in the group A.Conclusion: PAAF can induce intestinal harm.