N－甲基－D－天门冬氨酸受体拮抗剂对猫创伤性脑水肿的影响

The role of NMDA receptor antagonist on traumatic brain edema in cats

本实验观察了竞争性Ｎ－甲基－Ｄ－天门冬氨酸（ＮＭＤＡ）受体拮抗剂Ｄ－（－）－２－氨基－７－磷庚酸酯（Ｄ－ＡＰ７）对猫创伤性脑水肿的影响。结果发现Ｄ－ＡＰ７可明显减轻脑含水量，改善脑Ｎａ＋、Ｋ＋、ｃａ＋＋、Ｍｇ＋＋含量，提示兴奋性氨基酸与创伤性脑水肿有关，其中ＮＭＤＡ受体可能起主要作用，本文探讨了ＮＭＤＡ受体拮抗剂对创伤性脑水肿的保护机理。

Abstract The protective effect of D-AP7 (D-(-)-2-Amino-7-Phosphono-heptanoic Acid).a specific antagonist of N-Methyl-D-Aspartate (NMDA) receptor, was evaluated in cat with traumatic brain edema D-AP7 (15μg/kg) was administered through cisterma cerebellomedullaris. 20 min after head injury in one group (n=9).treated with artificial cerebrospinal fluid in another group (ACSF,n=9) and same operation (n=9) controls.After 6 hours all cats were sacrificed and the water.sodium .potassium.calcium, and magnesium content of the damaged hemispheres were evaluated. In D-AP7 group the brain water content was 77. 08± 0.40%, in ACSF group was 78.38 ± 0. 3.6% and 76. 27 ± 0.18% in same operation controls (P<0.01).D-AP7 treatment also increased the tissue potassium.magnesium and decreased the tissue sodium.calcium. The results suggest that excitatory amino acids are involved in the development of traumatic brain edema.NMDA antagonists may be of benefit in treating acute head injury .