戊糖乳杆菌31-1菌株产细菌素发酵条件优化

Optimization of Bacteriocin Produced by Lactobacillus pentosus 31-1

对戊糖乳杆菌31-1产细菌素的条件进行了优化,分别研究了培养温度,培养基起始pH值,培养基碳源、氮源,刺激因子等因素对细菌素产量的影响。组合因素优化结果得到最佳培养基与培养条件为:乳糖30g、胰胨15g、豆胨20g、牛肉膏30g、蛋白胨20g、吐温80 1mL、磷酸氢二钾2g、乙酸钠5g、柠檬酸铵2g、硫酸镁0.58g、硫酸锰0.25g,蒸馏水定容至1000mL,30℃培养24h,培养起始pH为6.5。在此条件下培养细菌素效价可达到640AU/mL,与起始培养基相比细菌素产量提高了8倍。

To optimize bacteriocin production,researching was done on the respect of incubation condition and the media components. And the optimum incubation time was 24h, the optimum temperature was 30℃, the optimum broth initial pH was 6.5. The optimum media component was: lactose 30g, trypone 15g, soybean peptone 20g, meat extract 30g, peptonethe 20g, Tween 80 1mL, K_(2)HPO_(4 )2g, NaAc 5g, Tri-Ammonium citrate 2g, (MgSO_(4)) 0.58g, MnSO_(4) 0.25g, H_(2)O 1000mL. Production of bactericoin under the optimum broth was 640AU/mL, 8 times than MRS media.