密纹片姜茎尖培养脱毒快繁技术研究

Virus Removal and Rapid Propagation of Miwen Zingiberofficinale Rosc

CMV和TMV病毒的侵染导致密纹片姜产量降低,品质下降,通过茎尖脱毒培养和筛选获得脱毒苗并建立快繁体系,以提高产量,改善品质。茎尖接种培养基为MS+6-BA1.0￣3.0mg/L+NAA0.1mg/L,丛生芽快繁培养基为MS+6-BA2.0￣4.0mg/L,生根培养基为1/2MS。经过一年多的研究和培养,获得大量脱毒苗并应用于生产。本方法也适用于黄姜类其它品种的脱毒和快速繁殖。

Virus diseases cause the reduction in the yield and quality of Zingiber officinale cv.miwen.Through de-virus and repid propagation by stem apices tissue cuiture,the plantlets were gotten successfully free from virus,with hope to improve rts yield and quality.Stemtip medium is MS+6-BA1.0~3.0 mg/L+NAA0.1 mg/L,Medium used for producing rosette buds is MS+2.0-4.0 mg/L. Rooting medium is 1/2MS.