玉米叶绿素含量测定方法研究

Determined Methods of Chlorophyll from Maize

以玉米幼苗绿叶为试材,研究了6种叶绿素提取液的吸收光谱和提取效率及低温冷冻对叶绿素提取的影响,结果表明:6种叶绿素提取液的吸收光谱基本相似,可以沿用Arnon法的计算公式进行叶绿素含量的测定;室温下,浸提法要优于研磨法,混合液浸提叶绿素比单独用丙酮或乙醇浸提要快,其中丙酮乙醇(水)配伍提取速度较快而稳定,是最佳的叶绿素提取方法。叶片冷冻后快速浸提确实能加速叶绿素提取速度,但叶绿素易受光热破坏,故应注意遮光。

The absorption spectrums and extraction efficiencies of chlorophyll from maize seedling after frozen treatment were compared on six determined methods using different organic solvents. The results showed that the absorption spectrums of five solutions were identical with that of acetone solution, so Arnon's formula of chlorophyll was suitable for other methods. In house temperature, soaking methods were better than Arnon method. The extraction efficiencies of chlorophyll using mixture solution better than using acetone or ethanol alone, methods of acetone mixed ethanol were preferable for fast extraction and stability. Soaking methods in 50℃ after frozen treatment could accelerate extraction of chlorophyll, but also speed up degradation of chlorophyll, so light screening was necessary.