DNA抗DNA免疫复合物促进肾小球系膜细胞产生白介素6

DNA — anti — DNA IMMUNE COMPLEXES ENHANCE IL— 6 PRODUCTION BY MOUSE MESANQIAL CELLS

应用白介素6(IL-6)依赖细胞磉KD83增殖试验,观察了狼疮性肾炎患者血清中提取的DNA抗DNA免疫复合物(DNA-anti-DNA IC)对体外培养的小鼠肾小球系膜细胞产生IL-6的影响。结果表明,DNA-anti-DNA IgG、DNA-anti-DNAIgM、DNA-anti-DNA IgA均能促进系膜细胞产生IL-6,其中以DNA-anti-DNA IgM的作用最为明显;系膜细胞IL-6的产生与DNA-anti-DNA IC呈剂量依赖关系。

Using the IL—6 dependent hybridoma cell line KD83, we investigated the relationship between KNA—anti—DNA immune complexes(DNA—anti—DNAICs)from the sera of lupus nephritis patients and interleukin 6 (IL—6) production by mouse glomerular mesangial cells cultured in vitro. The results show that DNA—anti—DNA IgM and DNA—anti—DNA IgG and DNA—anti—DNA IgM all enhance IL—6 production by mesangial cells; of those DNA—anti—DNA IC, the effect of DNA—anti—DNA IgM on IL—6 production by mesangial cells was significant. This enhanced production of IL—6 by mesangial cells was dependent on concentration of DAN—anti—DNA ICs.