肾病综合征患儿末梢血中性粒细胞活性氧产生异常

ABNORMAL PRODUCTION OF REACTIVE OCYGEN SPECIES BY PERIPHERAL BLOOD POLYMORPHONUCLEAR NEUTROPHILS IN CHILDREN WITH NEPHROTIC SYNDRONE

我们观察了肾病综合征(肾综)患儿末梢血中性粒细胞(PMN)产生活性氧(ROS)情况和不同来源的PMN及血浆混合培养后ROS产生状况,结果表明肾综患儿PMN自发性ROS产生亢进,激发后ROS产生不足,缓解肾综患儿均恢复正常;不同组合的PMN培养提示肾综患者血浆中存在激活PMN物质是PMN自发性ROS产生过多的原因,而PMN长期处于高分泌状态是PMN激发后ROS合成减少的原因。本研究提示PMN产生ROS异常在肾综发病机制和免疫异常等方面起一定作用。

The production of reactive oxygen species(ROS) by peripheral blood polymorphonuclear neutrophils (PMN)of children with nephrotic syndrome(NS) and the production of ROS by mixed culture combined with plasmas of different donors and PMN of different donors were first examined. It was observed that the spontaneous production of ROS by PMN of children with NS was increased and the potent stimulated production of ROS was poor. But in remission production of ROS by PMN returned to normal. Mixed culture showed that some substances activating PMN in nephrotic plasmas caused the spontaneous production of ROS and that persistent hyper—production of ROS by PMN from nephrotic patients caused lack of stimulated production of ROS by PMN. These results suggested that the abnormal production of ROS hy PMN may play an important role in the pathogenesis of NS and in the changes of immune response in NS。