流行性出血热特异性循环免疫复合物的动态观察和分析

Observation and Analyses of Epidemic Hemorrhagic Fever Specific Circulating Immune Complexes

应用双抗体夹心ＥＬＩＳＡ检测了８８例流行性出血热患者２２８份血清中特异性循环免疫复合物，并与胶固素－ＥＬＩＳＡ检测循环免疫复合物的结果进行了比较。结果表明，胶固素－ＥＬＩＳＡ检出循环免疫复合物１８３份（８０．２６％），抗原特异性ＥＬＩＳＡ检出ＩｇＧ型循环免疫复合物１８８份（８２．４５％），ＩｇＭ循环免疫复合物２０５份（８９．９１％），ＩｇＡ型循环免疫复合物１４７份（６４．４７％）。在各类特异性循环免疫复合物中，ＩｇＭ型在各病期检出率最高，ＩｇＧ型次之，ＩｇＡ型最低。在发病初期，各类特异性循环免疫复合物检出率均较高，极期达高峰，随病情缓解而渐降低。对特异性循环免疫复合物检出率与流行性出血热患者病情程度相关性的观察表明，轻型患者检出率较中，重型患者为低，而中、重型间检出率差别不明显。此外，轻型患者特异性循环免疫复合物动态变化明显，极期达高峰后很快下降，而中、重型患者检出率下降趋势较缓慢。上述结果直接证实了流行性出血热循环免疫复合物的形成是特异性病毒抗原刺激机体免疫应答的结果，并构成了流行性出血热免疫发病机制中的重要环节。

Using an antigen-specific ELISA, epidemic hemorrhagic fever(EHF)-specific circulating immune complexes (CIC) were detected in 228 serum samples of EHF patients, the CIC sp positive rate detected by conglutinin ELISA was 80.36%,and the antigen-specific CICs of different immunoglobulin classes(IgG, IgM, IgA) were detected in 82.45%, 89.91%,and 64.47% of EHF sera, respectively. The detected rates of the antigen-specific CICs in different phases of EHF and the correlation between the positive rates of CICs and the severities of EHF patients' clinical manifestations have also been observed. Results suggest that the specific CICs may play an important role in the immunological pathogenesis of EHF.