用聚合酶链反应和放射免疫法检测肾移植术后人巨细胞病毒感染

Studies on Det6ction of Human Cytomegalovirus Infection after Renal Transplantation with Polymerase Chain Reaction and Radioimmunoassay

将５０例肾移植术后发热病人的尿标本接种细胞，用聚合酶链反应（ＰＣＲ）技术检测第１代细胞培养上清中的人巨细胞病毒（ＨＣＭＶ）ＤＮＡ。结果１９例为ＨＣＭＶＤＮＡ阳性，阳性率为３８．００％，用固相放射免疫法（ＲＩＡ）检测ＨＣＭＶ抗原，阳性的有１５例，阳性率为２７．７７％。将５４倒尿标本接种人胎肺二倍体单层细胞，分离到６株ＨＣＭＶ。用ＥＬＩＳＡ检测６５例病人单份血清的ＨＣＭＶ－ＩｇＭ抗体，阳性的有１７例，检测４５例病人双份血清ＩｇＧ抗体，１１例的第２份血清的ＨＣＭＶ－ＩｇＧ抗体滴度比第１份有４倍以上升高。上述结果表明，ＰＣＲ检测结果与ＲＩＡ的检测结果基本符合；病人血清抗体的阳性率与ＰＣＲ检测的阳性率基本符合。

The detection of human cytomegalovirus DNA in the first passage of cell culture inoculated with urine specimens of patients after renal transplantion by means of polymerase chain reaction was carried out, Positive result was demonstrated in 19 out of 50 cases studied with a positive rate of 38.00%.Solid-phase radioimmunoassay was applied for the detection of human cytomegalovirus antigen from the first passage of cell culture inoculated with urine specimens after renal transplantion, and 15(27.77%) of 54 cases showed positive result for human cytomegalovirus antigen.The urine samples of 54 cases were subjected to virus isolation by using human fetal diploid cell line. Six of them showed positive result, and all these 6 isolates were identified as human cytomegalovirus by solid-phase radioimmunoassay.A 4-fold or greater use in the titer of anti-human convalescent-phase sera showedpositive results for human cytomegalovirus-specific IgM by ELISA.