持续感染甲型肝炎病毒细胞株的建立和应用

Establishment and Application of Frhk-4 Cell Line Persistently Infected with Hepatitis A Virus

用甲型肝炎病毒（ＨＡＶ）ＨＭ１７５株感染恒河猴胚肾细胞系（Ｆｒｈｋ－４），经过连续传代培养，建立了一株持续感染ＨＡＶ的细胞株。通过１８６代的传代培养和制备ＨＡＶ抗原的实际应用，表明本株带毒培养细胞具有生长速度快、繁殖率高的特点，能大量表达ＨＡＶ抗原。１：４－１：６扩增培养，３～４天可以长成单层，培养７～１０天可以收获ＨＡＶ抗原，用于组装抗ＨＡＶＩｇＭ诊断试剂盒，该试剂盒与美国雅培公司（Ａｂｂｏｔｔ）试剂盒对照检测１４８份血清，总符合率９８．６５（１４６／１４８），与临床对照检测２１３６份病人血清，符合率为９８．９２％（２１１３／２１３６）。

A persistent infection of hepatitis A virus (HAV) strain HM-175 was established in a fetal rhesus monkey kidney cell line (Frhk-4).In the course of 118 serial passages,Frhk 4 cells grew rapidly and HAV antigen was produced in large amounts.The cell culture was passaged 1:4-1:6 every 4 days, and at 7-10 days after passage,the high titer of HAV antigen was obtained. With the antigen, a capture ELISA for detection of IgM to HAV was established. Compared with HAV EIA Kit from ABBOTT,the concordant rate was 98.65% (146 / 148).A total of 2136 sera from the patients with hepatitis were detected,the concordant rate with the clinical diagnosis was 98.92% (2113/2136).