重型病毒性肝炎中丁型肝炎病毒的检测

Studies on Hepatitis D Virus infection in Fulminant Hepatitis B Patients

为了探讨丁型肝炎病毒（ＨＤＶ）感染在重型病毒性肝炎中的作用，对北京佑安医院１９８０年至１９８９年收治的５４例急性和亚急性重型肝炎和３８例急性乙肝患者血清，应用国产ＨＤＶＥＬＩＳＡ试剂测定抗－ＨＤ、抗－ＨＤＩｇＭ和ＨＤＡｇ，应用斑点杂交技术测定ＨＤＶＲＮＡ。结果发现重型肝炎组ＨＯＶ－Ｍ检出率明显高于急性乙肝组（２７．８％比５．３％．Ｐ＜０．０５）。单独ＨＢＶ感染和ＨＤＶ／ＨＢＶ混合感染的重型肝炎患者均有较高的病死率。提示ＨＤＶ感染是重型肝炎中重要的病原学因素之一，ＨＤＶ与ＨＢＶ具有协同作用加重肝损害，导致肝衰竭。

In order to explore the role of HDV infection in fulminant hepatitis B, 54 patients with fulminant hepatitis B and 38 patients with acute hepatitis B were stuided for prevalence of anti-HD, anti-HD IgM and HDAg in serum with ELISA,and HDV RNA by dot- blot hybridization.The positive rates of HDV markers were significantly higher in fulminant hepatitis B than those in acute hepatitis B (27.8% vs 5.3% P<0.05).The positive rate of HDV RNA was 20.4%(11/54).The mortalities were both high in simple HBV group (51.3%)and HDV/HBV group 33.3%.The results showed that HB associated with HDV infection usually aggravated the liver lesion and more often promoted the development of liver failure.