压力对培养乳鼠心肌细胞缺血再灌注的影响

Influence of pressure on ischemic and reperfused cultured myocardial cells of neonate rat

本研究以培养乳鼠心肌细胞为实验模型,评价压力对心肌细胞代谢及结构的影响。结果显示。心肌细胞在缺氧3h及再复灌1h后压力组与对照组比较。ATP升高、LDH释放及[Ca^(++)]i降低,细胞水肿减轻。结果说明2kPa及4kPa压力不增加心肌细胞在缺血及复灌期的损害。

The present study will elucidate the influence of pressure on the metabolic functions and structure of the myocardial cells. The cultured neonate rat myocardial cells were taken as experimental models. The results showed that after a period of anoxia of 3 hours and reperfusion of 1 hour, in the pressure groups, the ATP levels were higher, the LDH release and the intracellular calcium were lower than that in control group. At the same time,in the pressure groups, cells recovered butter from oedematous aspect than its did in the control group. We concluded that pressures of 2kPa and 4kPa did not increase the myocardial cells damage during ischemia and reperfusion.