摘要
应用碱性磷酸酶免疫组织化学和电镜细胞化学实验方法，观察体外培养鼠成骨细胞骨生成过程中基质小泡的形成，分泌及诱导基质钙化的细胞学变化。证实成骨细胞在体外适宜环境下，同样具备骨生成的能力，其成骨过程与体内的膜内成骨过程相似。
Abstract The procedure of in vitro
osteogenesisand calcification of rat calvaria osteoblasts wasstudied by the methods of ALPase
immunohis-tochemistry and electron microscopic cyto-chemistry.The formation,secretion and
ma-trix mineralization functions of matrix vesicleswere observed.It was concluded that
os-teoblasts have the capacity to differentiate intoosteocyte and formed calcified bone tissue in
a suitable enviroment in vitro.The calcificationprocedure is similar to that of intramembra- nous
ossification in vivo. For clinical rele-vance,the results will play an important rolein the treatment
of osteoporosis and long dis-tance bone defects.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1994年第2期97-98,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金
