抗T淋巴细胞单克隆抗体与丝裂霉素偶联的方法学研究

Effect of Domestic Monoclonal Antibodies on Treating Hosts Rejection and Prolonging Grafts lives.I.Methodological Experimentin Anti-T Lymphocyte Monoclonal AntibodiesCoupling with Mitomycin

我们采用抗Ｔ细胞单克隆抗体Ｔ３、Ｔ４，与具有抑制细胞ＤＮＡ合成的药物丝裂霉素交联，形成免疫偶联物，以期在临床应用中获得特异性的、对机体无副作用的、能选择性地作用于免疫活性细胞、延长移植物存活的效果。交联后的免疫偶联物，经过洗脱曲线，紫外扫描以及抗体效价测定等，实验结果表明单克隆抗体Ｔ３、Ｔ４和交联物葡聚糖Ｔ－４０与丝裂霉素Ｍｉｔｏｍｙｃｉｎ交联后的免疫复合物，抗体效价和药物活性均无明显影响，体外淋巴细胞转化试验表明交联后的免疫偶朕物具有很强的免疫抑制作用。

Abstract This paper reports a immunologic com-pound was produced by coupling of anti-TLymphocyte monoclonal antibodies(OKT3,OKT4)with mitomycin with the help of cou-pling agent dextran T-40,in order to act spe-cialized immunocompetent cells and prolongallograft survival without side-effect.The cou-pling compound was assessed by washingcurve,ultraviolet scanning and antibody titerdetermining. The results showed that the com-pound has no influence on antibodies(OKT3and OKT4)titer and mitomycin activity.Thelymphocyte transforming test in vitro indicat-ed that the compound had a stronger immuno-suppressive effect.