大鼠肝细胞、Kupffer和Ito细胞的分离与培养

Isolation and cultivation of liver,Kupfferand Ito cells of rat

我们采用Ｃｏｌｌａｇｅｎａｓｅ体内门静脉灌注结合体外Ｔｒｙｐｓｉｎ皿内消化制备游离肝细胞，进而采用ＰｒｏｎａｓｅＥ和Ｎｙｃｏｃｌｅｎｚ离心处理获单－Ｋｕｐｆｆｅｒ细胞和Ｉｔｏ细胞。三种细胞的产生率分别为１．２×１０８，１．１×１０７和２．３×１０７；其细胞纯度分别为９５％，８１％和８８％；存活率都在９０％以上。作者对三种细胞原代培养过程的形态特征和生物学活性进行了观察与讨论。

Abstract Perfusing the liver in vivo via the portalvein with collagenase and digesting it in vitroby Trypsin,the autor seperated the parenchy-mal liver cell.At the same time using thePronase E and centrifugation,we make out thenonparenchymal liver cells and further seper-ated and purified the Kupffer and Ito cells.The total numbers of three type cells were 1.2×108,1.1×107 and 2.3× 107; the purity ofthree type cells were 95%,81%and 88%.More than 90%of three type cells were vi-able. The questions about morphological fea-tures and biological function were discussed.