乙基硝基亚硝基胍诱发犬胃癌过程细胞动力学的研究

Studies on cell dinetics in canine stomach chromosomal 8q.carcinogenesis induced by N-Ethyl-N'-Nitro-N-Nitrosoguanidine

本研究利用实验犬胃癌模型，采用纤维胃镜活检，体外胃粘膜培养掺入３Ｈ－ＴｄＲ放射自显影方法，研究乙基硝基亚硝基肌（ＥＮＮＧ）诱发犬胃癌过程的细胞增殖动力学。结果表明，随着犬正常胃粘膜向癌前、癌发展，细胞标记指数（ＬＩ）出现三期变化，最初期下降，接着细胞ＬＩ不断提高，最终处于相对高值的平坦期（Ｐ＜０．０１）。Ｔｓ有延长趋势，但十分缓慢。Ｔｃ逐渐缩短。胃粘膜的增殖区逐渐扩大，显示细胞的去分化现象。

The cell kinetics was studied in the exper-imental ENNG-induced canine gastric cancerby autoradiographic method with tritiated invitro incubated biopsy speciments obtainedfrom lesions through a serial gastroscopic ex-aminations.The results showed that,with ca-nine background mucosa developing to cancer,there was an alteration of LI in the form of aninitial reduction,an increase phase and aplateau phase with a high value.The S-phaseduration showed a tendency to be prolonged,but it appeared slow and seemed to be rela-tively constant.The Tc became shorter withthe mucosal changes progressing from normalbackground to cancer.In the process of trans-formation, the proliferative zone of gastric ep-ithelial cell was shown to be enlarged gradual-ly. There is dedifferentiation phnenomenonwhich may be an important step in malignanttransformation.