生物流化床处理垃圾渗滤液的硝化强化实验研究

Study on nitrification bioaugmentation of landfill leachate in biofluidized bed reactor

采用厌氧/好氧/硝化耦合生物流化床反应器处理高浓度难降解垃圾渗滤液,通过摇瓶富集与开放体系扩大培养得到高浓度的硝化菌液,用于硝化生物流化床反应器的挂膜启动、驯化与动态运行实验。结果表明,扩培菌液中亚硝化细菌与硝化细菌的浓度分别达到9 .0×107 和3. 5×107 MPN/mL。硝化生物流化床的强化挂膜启动与驯化约历时30d,实际废水动态运行的结果显示,当进水垃圾渗滤液平均氨氮浓度为284 .4mg/L时,出水氨氮浓度为14. 3mg/L,达到了GB16889 1997一级排放标准,经过硝化生物强化的流化床反应器处理高浓度垃圾渗滤液的硝化速率高达28. 1gNH+4 N/m3·h,与未经生物强化的同类系统相比高出近1倍。

The refractory landfill leachate with high concentration was treated in an anaerobic/aerobic/nitrification biofluidized bed reactor system in this study.Nitrifying bacteria enriched by open-culture flask-shaker were used to bioaugment the nitrifying biofilm growth in reactor during the start-up and acclimation periods and further more to bioaugment the dynamic process in nitrifying biofluidized bed reactor. The result showed that the concentration of nitrite bacteria and nitrate bacteria in broth could achieve 9.0×107 and 3.5×107 MPN/mL respectively after enrichment.The start-up and acclimation periods lasted about 30 d and then the reactor turned into dynamic process with continuous leachate feeding.With influent ammonia nitrogen concentration of 284.4 mg/L,the effluent ammonia of the nitrifying biofluidized bed reactor is 14.3 mg/L and the effluent quality could meet the first control standard of GB 16889-1997 in China.Comparing with no bioaugmentation, the nitrification ratio of the nitrifying biofluidized reactor is twice as many and could achieve 28.1 g NH+_4-N/m3·h.