微生物絮凝剂产生菌的培养条件研究

Study on culture conditions of a bio-flocculant-producing strain

从活性污泥中分离筛选到1株高效絮凝剂产生菌E-9,初步鉴定其为大肠杆菌.首次发现大肠杆菌能产生微生物絮凝剂.该菌产生絮凝剂的适宜培养条件为:乙醇为碳源,大豆粉为氮源,初始pH值为6.5～7.2、温度为30℃.絮凝实验结果表明,该菌产絮凝剂对高岭土悬浊液的絮凝率可达97%～99.5%,而絮凝剂投加量仅为通用发酵培养基的1/15,絮凝效果优于聚丙烯酰胺等常用絮凝剂.

A bacterial strain designated as E.coli and named E-9 which can produce bio-flocculant with high flocculanting activity was screened from activated sludge .It is the first time to find that E.coli producing bioflocculant. The optimum culture conditions:alcohol is the optimum carbon source, soybean powder is the optimum nitrogen source, initial pH 6.5~7.2, 30 ℃.The results of flocculation test show that the E-9 can produce a strong-activity flocculants, which has higher flocculating effect compared with other ordinary flocculants, with flocculating rate of 97%~99.5% against kaolin clay suspension, and its dose is about 1/15 of the ferment media.