新生大鼠睾丸组织冷冻保存的研究

Experimental study on cryopreservationof neonatal testicular tissue ofras.

采用新生ＳＤ大鼠的睾丸组织为组织来源，研究睾丸组织的冷冻保存方法。结果表明，用二甲基亚砜作低温保护剂，４℃渗透平衡３０～４５ｍｉｎ，以０．４℃／ｍｉｎ的速率降温至－８０℃后并贮存于－８０℃低温冰箱内和４０℃水浴中１分钟快速复温的冷冻保存方法，冻存１个月组和冻存３个月组的睾丸组织在体外组织培养条件下，都具有良好的合成和释放睾酮的能力；将冻存１个月和冻存３个月后的睾丸组织移植到去势成龄雄性ＳＤ大鼠体内，移植物全部成活，且具有生长发育和分泌睾酮的机能。实验结果为人类胎儿睾丸组织的冷冻保存提供了重要的实验基础。

The techniques for freezing neonatal tes- ticular fragments and methods of viability as-says for preserved testicular tissues were stud-ied.The results showed that the testicular tis-sues of neonatal rats could be preserved at -800℃for three months at least with the cryop-reservation techniques of slowly freezing andrapidly thawing,the preserved tissues stillmaintained both their histologic integrity andtheir ability to produce detective testosteronein vitro and in vivo. It does found an importantexprimental basis on cryopreservation of hu- man fetal testicular tissues. The injury effectsof cryopretective agent on the testicular tissueare discussed.