L—谷胺酸改良处理猪主动脉瓣与瓣膜“内皮化”的研究

Experimental study of modified porcine valve treated by-I-glutamic acid and endothelialized in vitro.

我们将经过戊二醛（ＧＡ）和ＧＡ＋环氧氯丙烷（ＥＣ）化学改性处理的猪主动脉瓣，再用Ｌ—谷胺酸处理以后进行ＳＤ乳鼠主动脉内皮细胞种植，并与单纯ＧＡ瓣及未经ＧＡ处理的瓣叶做对比观察。结果发现：单纯ＧＡ瓣种植细胞未能存活，而经Ｌ—谷胺酸处理瓣叶却获得种植细胞规律性生长、增殖，两周后完全覆盖瓣叶表面。电镜观察及第Ⅷ因子染色证实，瓣小叶表面生长的内皮细胞中夹杂着大量成纤维细胞生长。与种植前培育细胞纯化及种植以后成纤维细胞控制不够有关。

The present study aimed to clarify the promising inhibiting effect of L-Glutamic acid on GA or GA+EC preserved porcine valves, and illustrate the processes of the leaflets surface covered by SD sucking rat's aortic endothelial cells. The porcine aortic valves were treated in following 4 groups: 1 ) 0. 5% GA treated valves, 2) none special biochemical treated valves, 3) valves treated by 0. 5% GA and then in 8% L-Glutamic acid solution, 4) 0. 5% GA for 72 hrs then 4% EC for 48 hrs before stored in the solution of 8% L- Glutamic acid. Counting seeded-cells from valve leaflets; Identification of cellular type by factor Ⅷ rAG; and electronmicroscopic assessment after endothelial cells were seeded in 1, 3, 7, and 14 days. The result showed: All the leaflets of group 2, 3, and 4 could acquire the endothelial cells and fibroblasts covering during 7-14 days. The covering rate in group 2 exhibited more than 80% after 7 days but in group 1 no viable cells could be seen in 2-3 days after implanting. The study revealed that the toxicity of GA in the heterografts were inhibited markedly by L-Glutamic acid and their biocompatibility were improved that make sucking rat aortic endothelium could grow regularly on the surface of bioprosthetic valves.