甲基强的松龙对家兔肝缺血再灌注损伤的保护作用

The beneficial effects of methylpred-nisolone on hepatic ischemlc-reperfusion injury in rabbits

作者测定了各组动物肝缺血再灌注前后肝组织及血中丙二醛（ＭＤＡ），谷胱甘肽过氧化物酶（ＧＳＨ－ｐｘ）及超氧化物歧化酶（ＳＯＤ）的变化，并行组织学、硝酸镧示踪和超微结构观察。结果表明：肝缺血再灌注后有氧自由基的产生及抗氧化酶的抑制，并发生缺血再灌注损伤。白细胞浸润、氧自由基的产生及肝窦内皮细胞，Ｋｕｐｆｆｅｒ细胞的损伤可能在这一过程中起重要作用。ＭＰ可减轻这种损伤。

This study investigated the effects of pre-treatment with methylprednisolone(MP)onliver tissue injury associated with hepatic is-chemia-reperfusion. Twenty-nine rabbits wererandomized into three groups. Group A re-ceived saline as a control.Group B was pro-cessed in a sham operation. Group C was pre-treated with MP(30～40mg/Kg).After onehour of hepatic reperfusion which followedForty-five minutes of complete ischemia,a sig-nificant increase in the level of liver tissueMDA(P<0.01),and a decrease in tissueGSH-Px(P<0.01)and blood SOD activities (P<0.01)occured .After pretreating the rab-bits With MP,the devation of MDA(P<0.01)was suppressed .during the period of reperfusion following ischemia. Histopatholo-gical and ultrastructural examinations showedan infiltration of a great number of neu-trophilic leukocytes,increased permeability ofcell membrane,and damage of liver cells,espe-cially that of epithelial and Kupffer cells.MPcould significantly lessened the above tissueinjuries.These findings suggest that hepaticischemia-reperfusion cause liver tissue injury.Infiltration of leukocytes,oxygen-free radi-cals,and,damage of epithelial and Kupffercells may play an important role in hepatic is-chemic-reperfusion injury. Pretreatment withMP has a protective effect on this type of tis-sue injury.