正常人肝细胞膜HBV－PHSA受体相关抗原及特性

STUDY ON THE HBV PHSA RECEPTOR ASSOCIATED ANTIGENS AND ITS CHARACTERISTICS

用蔗糖密度梯度离心法纯化ＰＨＳＡ受体阳性的正常人肝细胞膜（ＨＰＭ），通过Ｗｅｓｔｅｒｎｂｌｏｔ技术发现抗ＨＢＶＰｒｅＳ２（１２０～１４５）合成肽多克隆抗体可识别到两种抗原成份（７０ｋＤ和１２０ｋＤ）。用蛋白酶Ｋ、脂肪酶、神经氨酸酶消化ＨＰＭ后再进行Ｗｅｓｔｅｒｎｂｌｏｔ识别，结果表明这两种抗原成份分别具有糖脂蛋白和糖蛋白的性质。由于已知抗ＰｒｅＳ２（１２０～１４５）是针对ＨＢＶＰＨＳＡ受体抗原表位的抗体，故作者认为ＨＰＭ上这两种不同性质的ＨＢＶＰＨＳＡ受体相关抗原可能与肝细胞的ＰＨＳＡ受体密切相关，乙肝病毒ＰＨＳＡ受体与正常人肝细胞ＰＨＳＡ受体之间可能存在一定程度的结构相似性。

e purified the normal human hepatocyte plasma membrane (HPM ) with sucrose gradient centrifugationsThe hepatocytes had been previously tested as PHSA receptor positive. We found the polyclonal antibody againstPres2(120～145)synthetic peptide,which was identified against HBV PHSA receptor antigen epitopes,recog-nized two antigens of the normal human HPM with Western blot. Molecular weight of the recognized antigens was70kD and 120kD respectively. As a control, the SP2/0 cells which didn′t express PHSA receptor was not recog-nized by the anti-PreS2 ( 120～145).Further experiment results suggested a glycoprotein nature for the 120kDantigen and a glycolipidprotein nature for the other one. We suggest that these two antigens possessing a distinctchemical nature most relate to the PHSA receptor on normal human HPM which also expressed two classes ofbinding site.Our studies would be useful to further characterize the PHSA receptor of human hepatocytes.It wasalso important to realize the immunopathogenesis of HBV and modify the hepatitis B vaccine.