γ－干扰素在淋巴细胞介导的子宫颈角化上皮细胞毒作用中的意义

THE SIGNIFICANCE OF IFN GAMMA IN CYTOTOXICITY MEDIATED BY LYMPHOCYTES TO CERVICAL KERATINOCYTES

本研究以子宫颈角化上皮为靶细胞，用试管培养技术及非同位素细胞毒性试验，探讨了干扰素（ＩＦＮ－γ）对淋巴细胞介导的对宫颈角化上皮细胞毒作用的影响及细胞间吸附分子１（ＩＣＡＭ－１）与白细胞功能相关抗原１（ＬＦＡ－１）在细胞毒作用中的意义。结果显示ＣａＳｋｉ、ＳｉＨａ、ＨｅＬａ、Ｗ１２及ＮＣｘ均为ＮＫ抵抗、ＬＡＫ敏感细胞。ＩＦＮ－γ预处理靶细胞明显增加ＬＡＫ细胞的杀伤活性，但对ＮＫ活性影响不大；ＩＦＮ－γ预处理效应细胞可增强ＬＡＫ细胞对上述靶细胞的溶解作用，但并不改变ＮＫ对靶细胞的选择性；抗ＩＣＡＭ－１与ＬＦＡ－１单克隆抗体能有效地降低ＬＡＫ细胞对靶细胞的杀伤作用，而抗ＨＬＡＡＢＣ及ＨＬＡＤＲ则无此作用。提示ＩＦＮ－γ仅对ＬＡＫ细胞介导的对宫颈角化上皮的细胞毒作用有明显增强作用，而对ＮＫ细胞无明显影响；ＩＣＡＭ－１－ＬＦＡ－１通路为ＬＡＫ细胞结合并溶解靶细胞的主要通路，且这种细胞毒作用是非ＭＨＣ限制的。

nvestigation was performed to determine the significance of IFN-γand the effects of ICAM-1 and LFA-1 incytotoxicity mediated by lymphocytes to cervical keratinocytes by using a co-culture system and non-isotopic cyto-toxic assay. It showed that CaSki、SiHa、HeLa、W12 and NCx were all NK-resistant and LAK-sensitive. IFN-γpre-treated target cells up-regulated the susceptibility to lysis by LAK , not by NK.The cytotoxicity effect of IFN-γpretreated LAK cells , but not pretreated was strongly promoted NK cells. Anti-ICAM-1 and anti-LFA-1 McAbsblocked the lysis to some extent by LAK,but the blockage did not occur in case of using anti-HLA ABC and anti-HLA DR.ICAM-1/LFA-1 was the main pathway of LAK to bind and to kill the cervical keratinocytes,and thecytotoxicity could not be restricted by MHC.