计算机辅助的细胞核测量技术在食管癌临床中的应用

The nucl ear morphometric anal ysis and its clinical val-ue in esopbageal cancer

作者利用计算机辅助的细胞核形态定量分析新技术，测定４２例有随访资料的食管癌手术患者癌细胞核平均面积（Ａ）和平均核圆形因子（ＮＲＦ），探讨其临床应用价值。结果显示：食管癌组Ａ和ＮＲＦ均显著大于正常对照组（Ｐ值均＜０．０５）；ＮＲＦ与术后生存期，局部淋巴结癌侵润有明显关系（Ｐ均＜０．０５）；而上述形态参数在患者性别、年龄、肿瘤部位、肿瘤长度、侵润深度上的差异均无显著性（Ｐ均＞０．０５）。

AbstractThe technique of computer-assisted morphometricanalysis had been utilized to assess the prognosis formany cancers. However,there has not been the reportabout application of this method in esophageal cancer.The nuclear area(A) and nuclear roundness factor(NRF)of epithelial cells of 42 esophageal cancers andten normal esophaguses were measured by morphome-try and the results were compared with the extent ofthe tumor(length,depth and lymph node metastasis),pathologic grades of the cancer,survival time of thepatients, and so on.A and NRF of epithelial cells ofesophageal cancer were significantly larger than thoseof normal esophagus (P<0.001 and P<0.05 respec-tively),In the esophageal cancer group,NRP of ep-ithelial cells of patients with short survival time weresignificantly larger than those of long survival time(P<0.05 ).NRF of epithelial cells of patients with lym-phatic node metastasis were significantly larger thanthose of non-metastasis(P<0.05).A and NRF of ep-ithelial cells were not related with tumor length,inva-sive depth,and position,also not related with patientsex and age. The results comfirmed that nuclear mor-phometry can play an important role in the diagnosis,differential diagnosis and prognosis of esophageal can- cer