应用流式细胞仪测定骨巨细胞瘤的DNA含量

Flow cytometric analysis of DNA in giant cell tumor ofbone

用流式细胞仪对４４例骨巨细胞瘤（ＧＣＴ）ＤＮＡ含量进行了分析，并对其中２例ＧＣＴ进行ＧＣＴ肿瘤相关抗原和ＤＮＡ含量双参数测定。结果４２例Ⅰ、Ⅱ级ＧＣＴ中有１６例异倍体，１例肺转移和２例Ⅲ级ＧＣＴ为异倍体。统计学分析表明：（１）ＧＣＴⅠ级与Ⅱ级间的增殖速度与异倍体比例均无显著性差异。（２）Ⅰ、Ⅱ级原发和术后复发ＧＣＴ之间无论是异倍体所占比例和增殖状况都有显著性差异。（３）Ⅰ、Ⅱ级ＧＣＴ经体外培养后细胞增殖加快，具有ＧＣＴ肿瘤细胞相关抗原的细胞比例增加。提示ＧＣＴⅠ、Ⅱ级间形态学上的差异还不能完全反映其内在本质；证明流式细胞术在临床上可对ＧＣＴ局部侵袭性评价提供客观线索和依据；支持ＧＣＴ中存在肿瘤细胞和非中瘤细胞成分，只有肿瘤细胞成份在体外培养中增殖存活。

AbstractThe DNA content of 44 giant cell tumors (GCTs)was determined by flow cytometry and simultaneousannalysis of cell surface antigens and cellular DNA wasperformed in two of these GCTs. Two grade Ⅲ GCTs,one with lung metastases, were all aneuploid. Out of42 grades Ⅰ and Ⅱ GCTs,16 were aneuploid, statisti-cally, there was no difference in proliferative activityand rate of aneuploidy between grade Ⅰ and grade ⅡGCTs, but significant difference between primary andlocal recurrent GCTs existed. After 5 passages in cul-ture the proliferative rate of cells in 13 GCTs increasedsignificantly, the rate of cells bearing GCT associatedantigen stepped up and in 2 GCTs tested specially. Allthese findings demonstrate : (1) the morphological dif-ference of these tumors was not remarkable (2) DNAdeterminations as an adjunct to conventionalhistopathologic assessment may provide objective clini-cally relevant information for prognosis, (3) tumor andnon-tumor cells co-exist in GCTs and only tumor cellscould survive in long-term culture in vitro.