摘要
应用ras癌基因产物p21 ̄ras免疫组化染色的图象分析技术、流式细胞分析技术分别测定83例结直肠癌的p21 ̄ras平均光密度值(p21D)和DNA指数(DI),结合临床随访和病理学资料探讨其预后价值。结果提示:结直肠癌的p21D明显高于正常粘膜(P<0.001),术后生存≥5年组的p21D明显低于<5年组(P<0.001)。高p21D组患者预后较低p21D组差。二倍体肿瘤及低p21D异倍体肿瘤患者预后较高异倍体肿瘤患者明显好。Dukes′c期的p21D高于B和A期。表明ras癌基因表达与结直肠癌病程进展及转归有关,测定结直肠癌的p21 ̄ras的表达和DNA倍体将有助于预后判断。
Abstract83 colorectal cancer patients who underwent radi-cal surgery were studied.Serial sections were carriedout for detection of p21 ̄ras expression level by means ofimmunohistochemical assay combined with image anal-ysis technique, measurement of DNA ploidy by flow-cytometry and re-confirmation of dignosis.We foundthat the high level of p21 ̄ras expression indicated agroup of tumors with more aggressive behavior andworse prognosis. The five-year suvival rate was only26.83%in patients with p21D≥0.24 tumor,comparedwith 78. 57%in those with p21D<0.24 tumor. Five-year survivors tended to disply diploid and had lowerp21D. Detection of p21 ̄ras expression level(p21D)andDNA index in tumors may be helpful in predicting theoutcome of colorectal cancer patients.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1994年第8期480-482,共3页
Chinese Journal of Surgery