结直肠癌p21^（ras）的计量分析与预后

p21￣ras expression,DNA ploidy and prognosis in colorec-tal cancer

应用ｒａｓ癌基因产物ｐ２１￣ｒａｓ免疫组化染色的图象分析技术、流式细胞分析技术分别测定８３例结直肠癌的ｐ２１￣ｒａｓ平均光密度值（ｐ２１Ｄ）和ＤＮＡ指数（ＤＩ），结合临床随访和病理学资料探讨其预后价值。结果提示：结直肠癌的ｐ２１Ｄ明显高于正常粘膜（Ｐ＜０．００１），术后生存≥５年组的ｐ２１Ｄ明显低于＜５年组（Ｐ＜０．００１）。高ｐ２１Ｄ组患者预后较低ｐ２１Ｄ组差。二倍体肿瘤及低ｐ２１Ｄ异倍体肿瘤患者预后较高异倍体肿瘤患者明显好。Ｄｕｋｅｓ′ｃ期的ｐ２１Ｄ高于Ｂ和Ａ期。表明ｒａｓ癌基因表达与结直肠癌病程进展及转归有关，测定结直肠癌的ｐ２１￣ｒａｓ的表达和ＤＮＡ倍体将有助于预后判断。

Abstract83 colorectal cancer patients who underwent radi-cal surgery were studied.Serial sections were carriedout for detection of p21￣ras expression level by means ofimmunohistochemical assay combined with image anal-ysis technique, measurement of DNA ploidy by flow-cytometry and re-confirmation of dignosis.We foundthat the high level of p21￣ras expression indicated agroup of tumors with more aggressive behavior andworse prognosis. The five-year suvival rate was only26.83%in patients with p21D≥0.24 tumor,comparedwith 78. 57%in those with p21D<0.24 tumor. Five-year survivors tended to disply diploid and had lowerp21D. Detection of p21￣ras expression level(p21D)andDNA index in tumors may be helpful in predicting theoutcome of colorectal cancer patients.