清热祛湿方对肝纤维化大鼠肝组织SOD、MDA及超微结构的影响

Effect of “Qingre Qushi Decoction” on SOD, MDA and Ultrastructure of Liver Tissue in Liver Fibrosis Rats

探讨清热祛湿方对肝纤维化大鼠肝功能、肝组织SOD、MDA及超微结构的影响。采用复合因素诱导大鼠肝纤维化,光镜观察肝组织病理学变化、电镜观察肝组织的超微结构、并测定血清ALT、AST水平及肝组织SOD活性、Hyp、MDA含量。结果显示,清热祛湿方能明显改善大鼠肝组织病理变化和超微结构,降低模型大鼠血清ALT、AST水平以及肝组织Hyp、MDA的含量并提高肝组织SOD活性。提示,清热祛湿方可以有效抑制复合因素诱导的大鼠肝纤维化形成,其作用机制与抑制脂质过氧化、保护肝细胞,抑制肝纤维化的启动有关。

To discuss the effect of “Qingre Qushi Decoction” on liver function, SOD, MDA and ultrastructure of liver tissue in liver fibrosis induced by multiple factors, this Decoction was given to the rats. The serum ALT, AST, SOD activity, HYP and MDA contents were measured. Results: This Decoction could significantly ameliorate the liver tissue pathology and ultrastructure, decrease serum ALT and AST, HYP and MDA in liver tissue, and increase SOD activity. It is indicated that this Decoction can inhibit liver fibrosis possibly by suppressing lipid peroxide and protecting liver cell.