低温保存肝细胞移植救治实验性急性肝功能衰竭

Studies of long-term cryopreservation of hepatocytesand tkeir transplantation treating acute hepatic failurein wistar rats

本实验探讨了长期冻存肝细胞及其移植对急性肝功能衰竭的治疗作用。Ｗｉｓｔａｒ鼠肝细胞在液氮中冻存３０、６０、９０和１８０天，其活率分别为冻前细胞的７１．２％、７５．７％、６９．７％和７３．５％，各冻存组间无显著差异；活的肝细胞内的谷草转氨酶等酶含量在各组间均无显著差异，光镜及电镜显示细胞结构完整。Ｓｐｒａｇｕｅ－Ｄａｗｌｅｙ鼠同种同基因冻存肝细胞移植可将急性肝功能衰竭鼠的存活率从１／８提高到５／７（Ｐ＝０．０３５）．结果表明，肝细胞在液氮中保存，其活率并不随冻存时间的延长而降低，能保持活细胞结构完整、酶含量正常并在肝功能衰竭的治疗中起作用。

AbstractThe experiment aimed at studying long-term cry-opreservation of hepatocytes and therapeutic effect oftheir transplantation for acute hepatic failure. Hepato-cytes of wistar rats were cryopreserved in liquid nitro-gen for 30,60,90, and 180 days,Their viabilities wererespectively 71.2%, 75.7%, 69.7%, and 73.5%offresh cells’,however, there were no significant differ-ences among all cryopreserved groups. Differences ofviably intra-hepatocellular enzyme contents among allgroups, such as glutamic oxaIoacetic transaminase andso on,were not significant.Morphologically intacthepatocytes were observed under light microscopy andtransmission electron microscopy. Survival rate ofSprague-Dawley rats with acute hepatic failure were in-creased from 1/8 to 5/7 because of the transplantationof syngeneic frozen hepatocytes(p=0. 035 ). The re-suIts showed that after 30 days of cryopreservation inliquid nitrogen, the viability of hepatocytes decreasedsignificantly, but no Ionger decreased in subsequentfreeze up to 180 days. The viable intact cells and theirnormal enzyme contents were kept. The acute hepaticfailure could be treated by them.