聚合酶链反应诊断新生儿轮状病毒无症状感染的研究

DETECTION OF ROTAVIRUS FROM ASYMPTOMATIC NEONATAL INFECTION WITH POLYMERASE CHAIN REACTION

选用与Ａ组人类轮状病毒（ＨｕｍａｎＲｏｔａｖｉｒｕｓ，ＨＲＶ）编码ＶＰ７蛋白的第９（或８）基因片段中保守序列互补的两个引物，建立检测Ａ组ＨＲＶＲＮＡ的逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）技术，扩增产物片段约３４２ｂｐ。经检测３５例无腹泻症状新生儿１２９份粪便标本，并与聚丙烯酰胺凝胶电泳（ＰＡＧＥ）方法进行比较，结果前者检出ＨＲＶ核酸７７份（５９．６９％），而后者则检出４９份（３７．９８％），ＰＣＲ结果可能比较接近新生儿排泌轮状病毒的实际情况。

The primers selected in this study were complementary ones to the common conserved sequence of gene 9(or8 ) among A group four rotavirus serotypes.The method of polymerase chain reaction(PCR)was used for detec-tion of group A rotavirus nucleic acid in fecal samples.About 342 nucleotides were amplified with them.comparingwith polyacrylamide gel electrophorsis(PAGE ).We tested 129 fecal samples of 35 cases of asymptomatic rotavirusinfection of neonates by using reverse transcriptase-polymerase chain reaction(RT-PCR).of 129 fecal samplesfrom the 35 neonates ,77(59.69%)contained rotavirus RNA detected by RT-PCR,whereas only 49(37.98%)weredetected for rotavirus RNA by PAGE.The result probably were colse to the actual situation of the neonates shed-ding rotavirus.