肾综合征出血热病毒感染小鼠的抗体介导早死现象

ANTIBODY-MEDIATED EARLY DEATH IN MICE INFECTED WITH THE VIRUS OF HEMORRHAGIC FEVER WITW RENAL SYNDROME

本文报道被动输入免疫血清后感染ＨＦＲＳ病毒的成龄昆明系小鼠出现早死现象。每次实验将小鼠分为若干组，分别于感染前０．５，２４和４８小时ｉｖ注入不同量的兔或鼠抗ＨＦＲＳ病毒血清。对照组小鼠注入兔或鼠同量的正常血清。随后每只小鼠脑内感染１０５ＬＤ５０（０．０３ｍｌ）的Ａ－１６株病毒悬液。小鼠在感染前１天和感染后第１、２和４天，ｉｐ注射环磷酰胺，每次５０ｍｇ／ｋｇ。结果表明，注入免疫血清的实验组小鼠，其发病和死亡时间均早于对照组小鼠，尤以感染前２４小时注入免疫血清组为最，两者差异显著（Ｐ＜０．００１）。此外，实验组小鼠的脾脏肿大，脑髓的血管外周炎症病变较对照组严重。

The present report describes an experiment in which outbred adult Kumming strain mice were iv inoculatedwith HFRS virus-immune rabbit or murine serum.Control mice received nonimmune rabbit or murine serum.with different intervals of 0.5,24 and 48h,both groups of animals were i.c.infected with 105 LD50(0.03ml)of HFRS virus A-16 strain and injected with 50mg /kg cyclophosphamide dose/for four doses at days -1,+1,+2 and+4(day O :viral infection).Results showed that the incubation period was shorter than usual,and early deathphenomenon was observed in mice given small amount of immune serum,compared with control mice. In addition,in experimental animals,enlargement of spleens was noted and perivascular infiltration of inflammatory cells inbrain was more severe than those of control mice.The implications of these findings for the pathogenetic mecha-nism of HFRS are discussed.