丙型肝炎病毒基因组5’非编码区酶切分型研究

TYPING OF HEPATITIS C VIRUS GENOMES IN 5’NONE CODING REGION BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM

对１０５例ＨＣＶＲＮＡ阳性标本用Ｓａｕ３ＡⅠ和ＨａｅⅢ两种酶对其５’ＮＣ区扩增产物进行酶切。结果呈现４种酶切类型。对不同酶切类型的扩增产物进行序列分析，结果显示，不同酶切类型的扩增产物都源于ＨＣＶ，核酸序列中的酶切点与酶切结果完全吻合。对文献中已经确定基因型的３３株ＨＣＶ作了比较，分析了酶切类型与基因型的关系。发现对ＨＣＶ５’ＮＣ区－２９７至－４１片段ＰＣＲ扩增产物用这两种酶分别酶切，可以把ＨＣＶ按Ｃｈａｎ氏分型（１９９２）分为１、２、３三组。本文１０５例标本，１组（包括Ⅰ、Ⅱ型）占８２．９％，２组（包括Ⅲ、Ⅳ型）占１５．２％，１、２组混合占１．９％，未发现３组。其中以１组（Ⅱ型）为最多，但不超过６９．５％。

PCR products of 5 NC regfon of HCV genome were digested with Sau3AⅠand Hae Ⅲ,respectively in 105 casesof HCV RNA positive sera. Four patterns of restriction fragments were found.Sequence analysis revcaled that allPCR products were originated from HCV genome,no matter of their restriction fragment patterns. The restrictionsites of Sau3AⅠand Hae Ⅲin the sequence were identical.Sequence of 33 HCV isolates with known gene type inliterature were compared,and the relationship between restriction pattern and gene type were analysed.We foundthat HCV could be devided into 3 groups according to Chan’s classification by digcsting the PCR products of-294to -41 segment in HCV 5’NC region with Sau3A Ⅰand Hae Ⅲ,respectively. The incidence of HCV group 1(typesⅠ,Ⅲ) was 82.9%,group 2(typesⅢ,Ⅳ)was 15.2%,and group 1 and 2 mix was 1.9%in 105 cases of samples.No group 3 was found.Group 1(type Ⅲ)was at most,but not more than 69.5%.