摘要
对105例HCVRNA阳性标本用Sau3AⅠ和HaeⅢ两种酶对其5’NC区扩增产物进行酶切。结果呈现4种酶切类型。对不同酶切类型的扩增产物进行序列分析,结果显示,不同酶切类型的扩增产物都源于HCV,核酸序列中的酶切点与酶切结果完全吻合。对文献中已经确定基因型的33株HCV作了比较,分析了酶切类型与基因型的关系。发现对HCV5’NC区-297至-41片段PCR扩增产物用这两种酶分别酶切,可以把HCV按Chan氏分型(1992)分为1、2、3三组。本文105例标本,1组(包括Ⅰ、Ⅱ型)占82.9%,2组(包括Ⅲ、Ⅳ型)占15.2%,1、2组混合占1.9%,未发现3组。其中以1组(Ⅱ型)为最多,但不超过69.5%。
PCR products
of 5 NC regfon of HCV genome were digested with Sau3AⅠand Hae Ⅲ,respectively in 105
casesof HCV RNA positive sera. Four patterns of restriction fragments were found.Sequence
analysis revcaled that allPCR products were originated from HCV genome,no matter of their
restriction fragment patterns. The restrictionsites of Sau3AⅠand Hae Ⅲin the sequence were
identical.Sequence of 33 HCV isolates with known gene type inliterature were compared,and the
relationship between restriction pattern and gene type were analysed.We foundthat HCV could
be devided into 3 groups according to Chan’s classification by digcsting the PCR products
of-294to -41 segment in HCV 5’NC region with Sau3A Ⅰand Hae Ⅲ,respectively. The
incidence of HCV group 1(typesⅠ,Ⅲ) was 82.9%,group 2(typesⅢ,Ⅳ)was 15.2%,and group 1
and 2 mix was 1.9%in 105 cases of samples.No group 3 was found.Group 1(type Ⅲ)was at
most,but not more than 69.5%.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1994年第1期5-9,共5页
Chinese Journal of Microbiology and Immunology
关键词
丙型肝炎病毒
基因
酶切
分型
Hepatitis C virus
Restriction fragment length
polymorphism